α-modified eagle’s medium (α-mem) (Thermo Fisher)

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Thermo Fisher α-modified eagle’s medium (α-mem)
α Modified Eagle’s Medium (α Mem), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B1-modified+eagle+medium+(%CE%B1-mem)/%CE%B1+minimum+essential+medium+%CE%B1+mem/pm40304399-40-32-34
Average 90 stars, based on 1 article reviews

α-modified eagle’s medium (α-mem) - by Bioz Stars, 2026-10

90/100 stars

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Article Title: AZD1080, a specific inhibitor of GSK-3β, inhibits stemness and malignancies in osteosarcoma cancer stem-like cells
Article Snippet: 143B cells were cultured in α-MEM containing 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) and 1% antibiotics (penicillin, 100 U/ml, streptomycin, 100 μg/ml; Gibco; Thermo Fisher Scientific, Inc.).

Article Title: Comparison of 5 BMPs for their chondrogenic potentials and microfracture-mediated cartilage repair using heparin/PEAD coacervate sustained release polymer.
Article Snippet: Briefly, trabecular bone was cored out using a curette or rongeur and then flushed with a medium containing α-MEM (12571-063, Gibco, ThermoFisher Scientific),1 % antibiotic-antimycotic (Invitrogen, CA, USA) using 18-gauge hypodermic needles.

Isolation:

Article Title: A phenotypic <i>in vitro</i> assay of mouse oocyte maturation identifies reversible blockers of meiotic progression for non-hormonal contraceptive discovery
Article Snippet: .. All the GV oocytes were then 196 allowed to recover in a dish with pre-equilibrated α-MEM+GlutaMAX (Thermo Fisher Scientific, 197 Waltham, MA, USA) / 0.5% PS / 0.3% bovine serum albumin (BSA; Sigma-Aldrich) (α198 MEM/PS/BSA) media supplemented with 2.5 μM milrinone for 1 h at 37°C in a humidified 199 atmosphere of 5% CO2 in air to recover from the isolation process while maintaining meiotic arrest 200 prior to experimental use. ..

Article Title: A complex phenotypic assay of mammalian oocyte maturation identifies compounds that block meiotic progression for non-hormonal contraceptive discovery
Article Snippet: .. All the GV oocytes were then allowed to recover in a dish with pre-equilibrated α-MEM+GlutaMAX (Thermo Fisher Scientific, Waltham, MA, USA)/0.5% PS /0.3% bovine serum albumin (BSA) (Sigma-Aldrich) (α-MEM/PS/BSA) media supplemented with 2.5LμM milrinone for 1 h at 37°C in a humidified atmosphere of 5% CO 2 in air to recover from the isolation process while maintaining meiotic arrest prior to experimental use. ..

Cell Culture:

Article Title: Hydrogen sulfide donor GYY4137 attenuates RANKL-induced osteoclast differentiation and multi-nucleation.
Article Snippet: RAW264.7 mouse macrophage/monocytes were purchased from American Type Culture Collection (Manassas, VA, USA). .. In the culture system, RAW264.7 cells were cultured in α-MEM (phenol red free) (Gibco BRL/Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS) (Equitech-Bio, Inc., Kerrville, TX, USA), 100U/mL penicillin, and 100 μg/ mL streptomycin (Gibco BRL/Invitrogen, Carlsbad, CA, USA) at 37°C in 5% CO2. ..

Recombinant:

Article Title: Letrozole Reduces Ovulatory Responsiveness in In Vitro‐Grown Mouse Follicles
Article Snippet: .. For follicle culture, we used phenol red‐free α‐MEM Gluta‐MAX (Thermo Fisher Scientific) supplemented with 0.1 IU/mL recombinant human FSH (rFSH, follistim, MSD, Tokyo, Japan), 5 μg/mL bovine insulin (Sigma‐Aldrich, St Louis, Missouri), 5 μg/mL human apo‐transferrin, 5 ng/mL sodium‐selenite, 5 ng/mL ascorbic acid (Sigma‐Aldrich), 2 mM sodium pyruvate (Wako, Osaka, Japan), and 5% charcoal dextran‐treated fetal bovine serum. .. Follicles were individually placed in ultra‐low attachment 96‐well plates (Corning, New York, NY, USA), filled with 200 μL/well of culture medium and without mineral oil.

FACS:

Article Title: Regenerative bone-targeted nanoparticles modulate osteoclast function
Article Snippet: To assess the effect of NP treatments on the bone resorption activity of osteoclasts, a bone resorption assay was performed using the Bone Resorption Assay Kit (Cosmo Bio). .. Briefly, bone resorption assay fluoresceinamine-labeled chondroitin sulfate (FACS) was added to the assay plate and incubated at 37 °C for 1 h. The assay plate was then washed with PBS, and bone marrow-derived cells were added to the wells in phenol red-free α-MEM (Gibco) supplemented with 10% FBS, 20 ng mL −1 M-CSF, and 40 ng mL −1 RANKL. .. Briefly, bone resorption assay fluoresceinamine-labeled chondroitin sulfate (FACS) was added to the assay plate and incubated at 37 °C for 1 h. The assay plate was then washed with PBS, and bone marrow-derived cells were added to the wells in phenol red-free α-MEM (Gibco) supplemented with 10% FBS, 20 ng mL −1 M-CSF, and 40 ng mL −1 RANKL.

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